BSc., MSc., PhD
Director, Center for Research,Innovation and Development (CRID), FUTO
Environmental/Social Safeguard Officer (ACE-FUELS) The World Bank [www.https//acefuels-futo.org/]
Head, Bioremediation Unit, Center of Excellence in Local Materials and Substitution [CE-LoMaS] FUTO
Professor Toochukwu Ekwutosi Ogbulie is aProfessor of Biotechnology and currently, the Director of Centre for Research, Innovation and Development(CRID) at the Federal University of Technology Owerri (FUTO). She holds a Doctor of Philosophy Degree in Biotechnology, M.Sc., and B.Sc. in Microbiology. She is a registered Environmental Specialist (RES, A306) with specialization in Bioenergy and Environment under National Registry of Environmental Professionals, NREP USA. She has a profound interest and experience in Environmental Biotechnology/ Renewable Energy, Food Biotechnology, Microbial Biotechnology and Bioinformatics. She has carried out several empirical and operational research in Biotechnology using a strong quantitative and analytical skills which she obtained from varied international merit-based certificate courses that she has attended including summer certificate Course in Molecular Biology Techniques for DNA Technology; Practical course in Bioinformatics” Computer Methods in Molecular Biology at International Centre for Genetic Engineering and Biotechnology (ICGEB-CEI), Trieste Italy; Course in Environmental Management as Registered Environmental Specialist by NREP , USA; Chineese Academy of Science CAS-TWAS Food Biotechnology Training, Beijing, China; Wellcome Trust Advance Course on Next Generation Sequencing in Sanger S e q u e n c i n g I n s t i t u t e Hinxton Cambridge UK; Wellcome Genome Campus Advanced Overseas Course on Human and Vertebrate Genomics in Durban , South Africa; ProBIOGAS International Biogas plant engineering and operating training in Steinbeis Institute of Management and Technology (SIMT) Filderhauptstraße by Internationales Biogas und Bioenergie Kompetenzzentrum [IBBK], Stuttgart Germany, Course on Energy Transition: Innovation Towards a Low Carbon Future, MOOC INSTITUT FRANCAIS DU PETROLE (IFP) Rueil- Malmaison, Paris, France, Course on Bacterial Genomes: From DNA to Protein Function Using Bioinformatics Wellcome Genome Campus Advanced Courses, Sanger Sequencing Institute, Wellcome Genome Campus, Hinxton Cambridge, United Kingdom, etc. She has used these skills to make a huge impact in Research and Development by yearly training of students and staff of universities and youth forums.
Interestingly, Prof. Mrs. Ogbulie and her Bioremediation research team have been in search of possible environmentally friendly strategies for cleanup operations in crude oil polluted agricultural soil. Currently the team has confirmed the possible identities of bacterial isolates with identical sequence of genes of negligible differences coding for biosurfactant production and hydrocarbon degrading genes required for degradation of crude oil using standard molecular biology/ OMICS- driven Techniques which paved way for the meritorious NRF-TETFund grant to her team. The team is on the verge of developing a construct to create a novel organism with composite degradative genes to be employed in monoculture and multi-culture operational systems during bioremediation.
Thinking out of box and considering the food security of her country, Prof. Mrs. Ogbulie has through the help of wonderful mentors of Innov8 and iFAIR, developed a prototype automated dispensing device- AquaRES- for maintenance of concrete and plastic fishpond water quality for improved fish farming, reducing the fish mortality rate hence, increased harvest.
Prof. Mrs Ogbulie was aformer Deputy Director, Centre for Entrepreneurial Studies (CES) and former Deputy Dean of Post graduate school, FUTO;a member of the University Senate; member of Management Team of African Centre of Excellence – Future Energies and Electrochemical Systems (ACE-FUELS) FUTO, Co-Chairman of Committee for establishment of Centre for Biotechnology and Molecular Biology (CBMB) which attracted TETFund /NUC approval. She has also served 3 terms, as the Head of Department of Biotechnology(2016 – 2019) in the University. She has ultimately contributed to research and Development in the University especially in the field of Biotechnology and the Environment and has supervised many post-graduate students. She is a member of some national and international organizations as Nigerian Society for Microbiology (NSM), Biotechnological Society of Nigeria (BSN), Nigeria Environmental Society (NES), Organization for Women in Science for the Developing World (OWSDW/ TWOWS), Society for Applied Microbiology (SFAM) UK, American Society for Microbiology (ASM), Federation of European Microbiologist (FEMS); Graduate Women in Science (GWIS) USA which she is currently the Vice president of the international chapter. Prof. Mrs Ogbulie has attended and made presentations in several scientific national and international conferences and has to her credit, authored and co-authored ninety-eight (98) scientific publications/ works made up of articles in peerreviewed journals and book chapters. She is married with five (5) lovely children.
Academic Qualification
PhD- Biotechnology
MSc. – Microbiology
BSc. – Microbiology
RES- Registered Environmental Specialist (RES-306)
Professional Qualifications
32) National Workshop on Patents, Trade Secrets, and the Patent Cooperation Treaty (PCT) -Effective
Utilization of the IP System by Universitiesand Research Institutions, Abuja, Nigeria 7th to 8th
November 2023.
31) Africa Higher Education Centers of Excellence (ACE) World Bank Project 10th ACE Impact
Regional Workshop organized by Association of African Universities and “The World Bank Group.”
29th. October – 4th November 2023, Abidjan, Cote d’Ivoire.
30) PATLIB seminar − Knowledge Transfer to Africa (KT2A) Organized by Federal Ministry of Industry,
Trade, and Investment Abuja in Collaboration with European Patent Office, Munich Germany, 5th to 6th
September 2023.
29)Africa Higher Education Centers of Excellence (ACE) World Bank Project 9th ACE Impact Regional
Workshop organized by Association of African Universities and “The World Bank Group” 29th.
May – 2nd June 2023, Marrakech, Morocco.
28) Workshop on Access to Technology for Innovation and the Development of Technology and
Innovation Support Centers (TISCS) in Nigeria Organized by the World Intellectual Property
Organization (WIPO) and the Patents & Designs Registry (Nigeria), UN House, Garki Abuja. 3-5th April
2023.
27)TETFund training on “Entrepreneurship and Innovation in Higher Education” at Rockview Royale,
Wuse 2 Abuja from March 22 to 24th 2023.
26) Honoris United Universities Certificate Course in Business/Entrepreneurial Skills. A 21st Century Skill
Certificate 29th August 2022 – 13th January 2023.
25) Africa Higher Education Centres of Excellence (ACE) World Bank Project 8th ACE Impact Regional
Workshop organized by Association of African Universities and “The World Bank Group” 14th
– 18th November 2022, Banjul The Gambia.
24) The UN International Year of Basic Sciences for Sustainable Development (IYBSSD) Roundtable 2022
organized by Academy of Science of South Africa (ASSAF), 25th August 2022.
23) Innovation Fellowship for Aspiring Investors and Researchers (i-FAIR), INNOV8 Hub, Organized by
Nigeria and State of Israel. Abuja. June 2nd – 10th June 2022.
22) Science Communication Training Workshop for Scientists and Regulators under the Africa Coalition
for Communicating about Genome Editing. Theme “Framing the Genome Editing Narrative” 16th – 19th
March 2022.
21) Innovation Fellowship for Aspiring Investors and Researchers (i-FAIR), INNOV8 Hub, Organized by
Nigeria and State of Israel. Abuja. 16th – 27th February 2022.
20) Innovation Fellowship for Aspiring Investors and Researchers (i-FAIR), INNOV8 Hub, Organized by
Nigeria and State of Israel. Abuja. 6th – 10th December 2021.
19) Elsevier workshop on “Writing without Plagiarism and Proper Citation using Mendeley, Organized
by Unversity of Lagos (UNILAG), July 28th, 2020
18) Clarivate WEB of Science workshop on “Research Evaluations using Web of Science” organized by
Association of African Universities, September 4th, 2020.
17) Clarivate WEB of Science workshop on “Research Evaluations using InCites program” organized
by Association of African Universities, September 11th, 2020.
16) Clarivate WEB of Science workshop on “Building Profile for Authors and Research Organizations”
organized by Association of African Universities, September 4th, 2020.
15) Course on Energy Transition: Innovation towards a Low Carbon Future, MOOC INSTITUT
FRANçAIS DU PéTROLE (IFP) Rueil- Malmaison, Paris, France 24th March – 6th of May 2019.
14) Training Workshop on “Teaching Skills and Talent Hunt in Entrepreneurship” Organized by
Centre for Entrepreneurial Studies (CES) FUTO, 14TH May 2019
13) Course on Bacterial Genomes: From DNA to Protein Function Using BioinformaticsWellcome
Genome Campus Advanced Courses and Scientific Conferences, implemented by Sanger Sequencing
Institute, Wellcome Genome Campus, Hinxton Cambridge, United Kingdom11th -26th June 2018
12) ProBIOGAS International Biogas plant engineering and operating training in Steinbeis Institute of
Management and Technology (SIMT) Filderhauptstraße 142, 70599 organized by Internationales Biogasund Bioenergie Kompetenzzentrum [IBBK], 19 – 27 September 2017 Stuttgart, Germany
11) Workshop on Biogas Technology and Development for Sustainable Energy, Organic Agriculture, and
Environmental Protection organized by The National Biotechnology Development Agency (NABDA)
Abuja, Nigeria 23rd – 25th August 2017.
10) Leadership training on the Fundamentals of Office Administration, Leadership Skills and Effective
communication: Processes and Practices, implemented by Center for Human Resource Development,
Federal University of Technology Owerri, Nigeria February 14th – 15th, 2017.
9) Wellcome Genome Campus Advanced Course on Next Generation Sequencing implemented by Sanger
Sequencing Institute, Wellcome Genome Campus, Hinxton Cambridge, United Kingdom. March 11th –
18th, 2016
8) OMICS MOUAU Preconference cum Hands on Training on Essential Analytical Methods in
Biotechnology at Centre for Molecular Bioscience and Biotechnology (CMBB), Michael Okpara
University of Agriculture Umudike, Abia State. Nigeria February 22nd – 27th, 2016
7) TWAS-ROESEAP International Workshop on Food Biotechnology “Synthetic Biology for
New/Improved Food Products: A solution to for Food Security” at Faculty of Science, Kasetsart
University, Bangkok Thailand. Dec 19-24th, 2016
6) TWASFood Biotechnology Training Course for DevelopingCountries implemented by The World
Academy of Science (TWAS), Chinese Academy of Science (CAS), and The Centre of Excellence for
Biotechnology (CoEBio), Beijing, China, Nov 24th – 30th 2015.
5) Short course on Environmental Management covering modules as Health Safety and Environment
management systems, Environmental Pollution issues in oil and gas Industry, Environmental Impact
Assessment methodologies and auditing and Health Impact assessment. -current trends.
Implemented by National Registry of Environmental Professionals, Lagos State, May 2013
4) Hands-on Molecular Biology and Biotechnology Training on Basic Molecular Biology Techniques and
Bioinformatics at Nigerian Institute of MedicalResearch Yaba, Lagos State, 3rd – 8th August 2009 and
2nd-7th August 2010.
3) National Registry of Environmental Professionals (NREP), USA. Course on Environmental Management
as Registered Environmental Professionals/Specialists, May 2008. Certificate obtained: Registered
Environmental Specialist (RES).
2) International Centre for Genetic Engineering and Biotechnology, (ICGEB), and Central European
Initiative(CEI), Trieste, Italy. Practical Course on Bioinformatics: Computer Methods in Molecular
Biology, June, 2008.
Certificate obtained: Certificate on Bioinformatics/Computer Methods in Molecular Biology
(ICGEB/CEI).
1) Biotechnology Center, University of Agriculture Abeokuta, Ogun State (UNAAB). October 2006 Summer
Certificate Course on Basic Molecular Biology Techniques for DNA Technology
Honours/Awards
1) The TETFUND 2020 NRF Research Grant Award of N21, 000,000.00 (Twenty – one million
naira) for a project titled “Bioremediation of Crude oil polluted soil sites using genetically.
engineered superbugs for enhanced faster clean-up operations in Nigeria.”[Completed]
2) TETFund 2020 Institutional Based Research grant of N1, 470,000.00 (One Million, Four Hundred
and Seventy Thousand naira) for research titled “Molecular typing, Purification and
Metabolic Engineering of yeasts and Lactic acid Bacteria (LAB) in palm wine for
application in food Industry”[Completed]
3) The TETFUND 2021 NRF Research Grant Award of N 30,934,580.00.00 (Thirty million, Nine
Hundred and Thirty-Four Thousand, Five Hundred and Eighty naira) for a project titled.
“Controlof Bacterial Diseases of Fish in Aquaculture Systems Using Indigenous Plant Materials”
4) Nigerian Content Development and Monitoring Board [NCDMB] 2021 grant award of Center of
Excellence in Local Materials and Substitution CE-LoMAS [PI for Bioremediation Section]
5) The World Bank group 2019 Award of African Center of Excellence in Future Energy and
Energy and Electrochemical Systems (ACE-FUELS) Federal University of Technology Owerri,
Nigeria [Team Member].
6) UK Bursary award to attend Wellcome Genome Campus Advanced Course on Human and Vertebrate
genomics implemented by Sanger Sequencing Institute, Hinxton Cambridge, United Kingdom in Snell
Parade Durban 4001, South Africa August 10th – 12th, 2017
7) TWAS-ROESEAP 2016 fellowship Award for International Workshop on Food Biotechnology “Synthetic
Biology for New/Improved Food Products: A solution to for Food Security” at Faculty of Science,
Kasetsart University, Bangkok Thailand. Dec, 2016.
8) UK Bursary Fellowship award to attendWellcome Genome Campus Advanced Courseon Next
Generation Sequencing implemented by Sanger Sequencing Institute, Wellcome Genome Campus,
Hinxton Cambridge, United Kingdom. March2016.
9) Fellowship Award by The World Academy of Science (TWAS) andChinese Academy of Science and in
collaboration with Centre of Excellence in Biotechnology China(CoEBio). Fellowship grant for course
on Food Biotechnology Training for Developing Countries, Beijing China, Nov – Dec2015
10). TETFUND bursary grant of Eight hundred and eighty thousand naira (₦880,000.00) awarded
by the Tertiary Education Trust Fund (TETFUND) of the Federal Ministry of Education, Nigeria,
to attend the 4th International Conference on Biodiversity, June 15-17, 2015, Las Vegas, Nevada USA
11) Best Poster Presenter Award by OMICS International conferences at the 4th International
Conference on Biodiversity, June 15-17, 2015, Las Vegas, Nevada USA.
12). Fellowship Award by International Centre for Genetic Engineering and Biotechnology
(ICGEB) in collaborationwiththe Centre for European Initiatives (CEI). Fellowship
grant for course on Bioinformatics: Computer Methods in Molecular Biology atthe
International Centre for Genetic Engineering and Biotechnology, (ICGEB) Trieste, Italy. June 2008.
Affiliations
Courses Assigned to Teach
Course code Title
BTC 201 Introductory Biotechnology I
BTC 202 Methods in Recombinant DNA technology I
BTC 306 Methods in Recombinant DNA technology II
BTC 409 Environmental Biotechnology
BTC 415 Analytical Methods and Quality control in Biotechnology
BTC 503 Industrial Biotechnology
BTC 509 Biotechnology Robotics and Analytical Methods.
BTC 504 Genetic Engineering
BTC 506 Nucleotide Sequence Analysis
BTC 515 Forensic Biotechnology
BTC 518 Food Biotechnology
BTC 804 Bioethics
BTC 805 Advanced Molecular Biology
BTC 807 Fermentation Technology
BTC 808 Bioinformatics
BTC 809 Advanced Genetic Engineering
BTC 810 Research Techniques in Biotechnology
BIO 818/906 Technical Writing and Presentation
BTC 902 Bioremediation
BTC 909 Environmental Impact Assessment
Experience
Teaching and Professional Experience Research Fellow 2001 – 2002 Pollution control Department Imo State Environmental Protection Agency (ISEPA), Owerri, Imo State. (NYSC).
Facilitator and Research Fellow 1999– 2010 Anthonio Van Leuwenhoek Center for the Advancement of Microbiology in Nigeria. Owerri, Imo State . Assistant Lecturer 2005 -2008 Federal University of Technology Owerri, Nigeria.
Lecturer II 2008- 2011 Federal University of Technology Owerri, Nigeria.
Lecturer I 2011 – 2014 Federal University of Technology Owerri, Nigeria
Senior Lecturer 2014 – 2017 Federal University of Technology Owerri, Nigeria
Associate Professor 2017- 2020 Federal University of Technology Owerri, Nigeria
Professor 2020- Date Federal University of Technology Owerri, Nigeria
Research Affiliate 2016 – Date Institute of Environmental Health and Environmental Justice FUTO
Ag Head of Department 2016-2019 Department of Biotechnology, Federal University of Technology Owerri
Environmental and Social 2018- Date Safeguards Officer African Centre of Excellence in Future Energies and Electrochemical Systems FUTO (ACE-FUELS FUTO) The World Bank
Associate Dean Post Graduate School, 2020- 2021
Deputy Director Center for Entrepreneurial Studies (CES) 2021- 2022
Director Center for Research and International Development (CRID) 2022-Date
Research and Development (R&D) Lead Instructor/Resource Person11th – 16thJuly 2023. Capacity Building Workshop cum Hands on Training on Molecular Biology and BioinformaticsOrganized by Young Medical Laboratory Scientist Forum (YMLSF) of Nigeria, in Collaboration with Everight Diagnostic and Laboratory Services LTD Owerri- Imo State
Instructor/Resource PersonMarch 2022 and29th2023. TETFund NRF Concept Note Sensitization Workshop Center for Research, Innovation and Development Federal University of Technology Owerri, Imo State
Instructor/Resource Person11th April 2023 TETFund NRF Concept Note Sensitization Workshop Center for Research and InnovationFederal University of Petroleum Resources Effurun, [FUPRE]Delta State.
Instructor/Resource Person24th-25thJanuary 2023 Workshop on Building Professional Research Profile Federal University of Technology Owerri
Instructor/Resource Person Nov18 – 23, 2022 Short Course on Energy Transition and Renewable Energy Technologies. University of Applied Science Engineering and Technology (USET), Banjul, The Gambia
Instructor/Resource Person16th September,2022 2nd Workshop on ‘Presentation of Draft 10-Year Regional Strategic Plan for FUTO.
Academic Mentor1000 Girls 1000 Future Programme, New York Academy of Science, USA 2020 – Date
Academic MentorJunior Academy programme New York Academy of Science, USA 2020 – Date
Lead Facilitator/Resource PersonJune 18-22, 2019, Hands on Training of Academic staff, lab technologist, Researchers, and students during 2nd Capacity building/Hands -on -Training on Analytical Methods in Biotechnology at Federal University of Technology Owerri [FUTO], Imo State.
Facilitator/Resource Person August 1-5, 2017, Hands on Training of Academic staff, lab technologist, Researchers, and students during 1st Hands -on -Training on Analytical Methods in Biotechnology, at Federal University of Technology Owerri [FUTO], Imo State.
Facilitator/Resource Person February 22-27, 2016, Hands on Training of Academic staff, lab technologist, Researchers, and students during 1st OMICS International preconference workshop cum Hands -on -Training on Analytical Methods in Biotechnology, at Michael Okpara University of Agriculture Umudike Abia State.
Facilitator/ Resource Person June 9-10, 2015. Hands on Training of Staff and Students of the Department of Microbiology, Madonna University, Elele, River State on Molecular Biology Analyses.
Facilitator/Resource Person February 18th-19th, 2011. Training of Students of the Department of Plant Science and Biotechnology Imo State University on Potential Exploitation of Plant Biotechnology for Sustainable Economic growth.
|
Research Interest
Areas of Research Interest
*Environmental/ Food Biotechnology and Bioinformatics
Area of Specialization
* Renewable Energy and Environment/ Microbial Biotechnology/Bioremediation
Publications
1. J.N Ogbulie, I.O.C. Obiajuru and T.E. Ogbulie (2003). Bacteria and Helminth bioload of cultured Channa obscuraJournal of Aquatic Sciences 18(2)93-99. http://dx.doi.org/10.4314/jas.v18i2.19949
2. J.N. Ogbulie,T.E. Ogbulie and N.P. Okpokwasili (2005). Microbial Succession and Deterioration of Treated and Untreated Rubber latex Nigeria Journal of Microbiology 19 (1-2): 605-610. www.nsmjournal.org
3. T.E. Ogbulie and T.C. Akujiobi (2006). Bacteriological Qualities of Bottled Water sold in Imo State, Nigeria. International Journal of Natural and Applied Science. 2(2): 122-128. https://www.ajol.info/index.php/ijonas/article/view/36103
4. M.I. Nwachukwu, N.C. Duru, T.E. Ogbulie, I.O.Nwachukwu and C.C. Ibe (2006) Assessment of the antimicrobial efficacy of some Nigerian Toothpaste. International Journal of Natural and Applied Science 2(3): 272-278. https://www.ajol.info/index.php/ijonas/article/view/36103
5. S.I. Okorondu, W. Braide, T.E. Ogbulie and C.O. Akujiobi (2006). Antimicrobial and phytochemical properties of some traditional spices. Nigerian Journal of Microbiology 20 (3): 1301- 1308. www.nsmjournal.org
6. T.E. Ogbulie and M.O.E. Iwuala (2006). Bioremediation as a Potential Key to Environmental Sanitation and Sustenance in the Tropics. A Review International Journal of Environmental Health and Human Development. 7(2): 17-33.
7. T.E. Ogbulie andI.G. Kalu (2006). Essence of Personal Monitoring Approaches in the Assessment of Day-to-Day Exposure to Chemical Substances. International Journal of Environmental Health and Human Development 7(1): 29-34.
8. T.E. Ogbulie, I.G. Kalu and M.O.E. Iwuala (2007). Health Implications of Urban Housing and Population Settlement Pattern in Nigeria and the Mitigating Factors. International Journal of Environmental Health and Human Development. 8(1):35-42.
9. F.C. Nwanebu, T.E. Ogbulie and Akujiobi, C.A. (2007). The Bacteriology of some Food products sold in Owerri, Nigeria. International Journal of Biotechnology and Applied Sciences 2(1): 63-68. http://www.ijobas.org
10. T.E. Ogbulie, I.G. Kalu and I.J. Ibe (2007). Effects of Traditional Plant Preservatives on the Microbial load and Shelf Life of Palm Wine. International Journal of Tropical Agriculture and Food Systems, 1(1): 68-75. https://www.ajol.info/index.php/ijotafs/article/view/40889
11. T.E. Ogbulie, Akujiobi T.C. and I.G. Kalu (2007). Assessment of the Physicochemical Quality of Commercial bottled water sold in Imo State, Nigeria. International Journal of Natural and Applied Science 3(1): 131-137. https://www.ajol.info/index.php/ijonas/article/view/36158
12. T.E. Ogbulie, J.N. Ogbulie, and H.O. Njoku (2007) Comparative study on the microbiology and shelf-life stability of palm wine from Elaeis guineensis and Raphia hookeri obtained from Okigwe, Nigeria. African Journal of Biotechnology 6(7): 914-922. https://doi.org/10.5897/AJB2007.000-2110
13. T.E. Ogbulie, J.N. Ogbulie and I. Umezuruike (2008). Biodegradation of detergents by aquatic bacterial flora from Otammiri River, Nigeria. African Journal of Biotechnology 7(6): 824-830. https://doi.org/10.5897/AJB07.796
14. T.E. Ogbulie and S.U.N. Anune (2008). Quality Evaluation of some branded and unbranded Ice cream sold in Imo State, Nigeria. International Journal of Tropical Agriculture and Food Systems 2(1): 83-88. http://dx.doi.org/10.4314/ijotafs.v2i1.40965
15.T.E. Ogbulie(2008). Quality assessment of some sachet water sold in Owerri Imo State, Nigeria. International Journal of Tropical Agriculture Food Systems 2(1): 34-38. http://dx.doi.org/10.4314/ijotafs.v2i1.40956
16. I.G. Kalu., T.E. Ogbulie and F.N.Opara (2008), Pattern of Multi-drug resistance Salmonella enterica sero var typhi isolates in Nigeria. African Journal of Biotechnology 7(21): 3817- 3820. https://doi.org/10.5897/AJB08.327
17. Ibe, I.J., Ogbulie, J.N. and Ogbulie, T.E. (2008), Bacteriological Quality of On- The-Shelf Paediatric Syrups and Hospital Infusions. Nigerian Journal of Microbiology 22(1): 1714- 1718. www.nsmjournals.org
18. Ogbulie, J.N., Duru, C.N., Awujo, N.C. and Ogbulie, T.E. (2009). Physicochemical Quality of Municipal Borehole Water in Imo State, Nigeria. Pakistan Journal of Medical Research48(1): 8-11. https://www.pakmedinet.com/author/Ogbulie+Tochukwu+Ekwutosi http://www.pakmedinet.com/15733
19. Ogbulie, T.E., Nwigwe, H.C., Iwuala, M.O.E and Okpokwasili, G.C. (2010) Study on the use of Monoculture and Multispecie systems in Bioaugumentation of crude oil Contaminated Agricultural soil. Nigerian Journal of Microbiology24(1): 2160-2167. www.nsmjournals.org
20. Ogbulie, T.E., Nwigwe, H.C., Okpokwasili, G.C. and Iwuala, M.O.E (2011)Comparative study on the effect of symbiotic interaction between plants and non-indigenous isolates on crude oil remediation. Analele Universitatii din Oradea – Fascicula Biologie 18(1): 15-22. http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2011-1.html
21. Ogbulie, T.E., Nwigwe, H.C and Anyadoh, S. O. (2011). Comparative Assessment of Bioload of Healthy and Diseased Oreochromis niloticus as means of Food Security. Analele Universitatii din Oradea – Fascicula Biologie 18(1): 10-14. http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2011-1.html
22. Ogbulie, T.E., Udensi, U.J. and Ezeji, E.U. (2011).Telfaira occidentalis aided Rhizoremediation of Agricultural soil subjected to different crude oil contamination levels. International Journal of Tropical Agriculture and Food Systems 5(1); 1-7. https://www.ajol.info/index.php/ijotafs/article/view/76080
23. Ogbulie, T.E. (2011) Prospects for attaining an acceptable Housing Standard in Urban Settlements in Nigeria. An Overview. International Journal of Environmental Health and Human Development 12(2): 39-44.
24. Ogbulie, T.E. and M.O.E. Iwuala (2011) Environmental Monitoring: Essence of Questionnaires and Models in Indirect Assessment of Exposure to Chemical Substances. International Journal of Environmental Health and Human Development 12(2): 45-50.
25. Ogbulie, T.E.(2011). Phytoextraction of Nickel, Chromium, Lead and Copper from Crude oil polluted Agricultural soil. International Journal of Tropical Agriculture and Food Systems. 5(2): 99-106. https://www.ajol.info/index.php/ijotafs/article/view/86542
26. Ogbulie, T. E. and H. O. Njoku (2011) Effects of Different Bioremediation Processes on degeradation of n- Alkanes in crude oil contaminated Agricultural soil. International Journal of Tropical Agriculture and Food Systems 5(4): 240- 250 https://www.ajol.info
27. Ogbulie, T. E. and R. Nnopuechi (2012) Studies on the Phytoremediation of Crude Oil Contaminated Soil and its Effect on the Growth rate of Test Plants. International Journal of Environmental Health and Human Development 13(1): 32 – 40.
28. Ogbulie, T. E., Ogoamaka A. I. and Udensi, J U. (2013).Plasmid profiling and similarities in identities of probable microbes isolated from crude oil contaminated agricultural soil. Analele Universitatii din Oradea – Fascicula Biologie 20(1): 7-11http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2013-1.html
29. Ogbulie, T. E., Okereke J. N. and Chinakwe, E. C. (2013). Phytostimulation of Agricultural Soil Differentially Contaminated with Crude oil, using Vigna unguiculata International Journal of Environmental Health and Human Development 14(2): 31-41.
30. I. G. Emeka-Nwabunnia, B. O. Ibeh and T. E. Ogbulie(2014) High HIV sero prevalence among students of institutions of higher education in Southeastern Nigeria. Asian Pacific Journal of Tropical Diseases. 4(2): 159-165. www.elsevier.com/locate/apjtd doi:10.1016/S2222-1808(14)60334-0
31. Ogomaka, I.A.; Dike-Ndudim, J.N, Ogbulie, T.E.; Dike, D. O, Nwokeji, M.C., Egbuobi, R. C. (2014). Urinary tract infection among teenagers in umudioka Orlu L. G. A. of Imo State, Nigeria. Scientific Research Journal 2(1):25-28. www.scirj.org
32. Ogbulie T. E, Nwanebu F. C. and Nwachukwu A. A. (2014) Assessment of the Total Petroleum Hydrocarbon content of Agricultural soil polluted with different volume of crude oil during Plant-Microbe Interaction. Annals of West University of Timisoara, ser. Biology XVII (1):13-24https://biologie.uvt.ro; https://biologie.uvt.ro/annals/vol_XVII_1.pdf
33. Ezeji, E. U., Ogbulie, T.E., Onwurah, I.N.E. and Ujowundu, C.O. (2014). Effects of Dichlorvos on Reduced Glutathione, Glutathione S-Transferase and Lipid Peroxidation of Egg Laying Hens. International Journal of Chemical and Biological Science 1(1): 46 – 49. https://www.semanticscholar.org
34. Ogbulie, T. E., Ukoma, A. A. and Iwuala, M. O. E. (2014). Evaluation of the impact of climate change and possible mitigating factors on enhanced food security in Nigeria. International Journal for Environmental Health and Human Development 15(1): 1-19
35. Nsofor, C. A., Ogbulie, T. E. and Nsofor, C. M (2014) Conjugation Efficiency of Human Antibiotic Resistant Escherichia coli Isolates from different Regions of Nigeria. Journal of Pharmaceutical and Biomedical Science 4(7): 638-644.www.jpbms.info
36. Ogbulie, T. E., Nsofor, C. A. and Nze, F. C. (2014) Bacteria species associated with ugba (Pentaclethra macrophylla) produced traditionally and in the laboratoryand the effect of fermentation on product of oligosaccharide hydrolysis. Nigerian Food Journal 32(2): 73-80. Hosted by Elsevier https://doi.org/10.1016/S0189-7241(15)30120-X www.sciencedirect.com/science/article/pii/S018972411530120X
37. Ogbulie, T. E., Uzomah, A. O. and Agbugba, M. N. (2014)Assessment of the safety of some on-the—shelf canned food products using PCR- based molecular technique. Nigerian Food Journal 32(2): 81-91 Hosted by Elsevierhttps://doi.org/10.1016/S0189-7241(15)30121-1 www.sciencedirect.com/science/article/pii/S018972411530120X
38. Ogbulie, T. E., Nwanjo, G. O. and Azuwike, C. O. (2015) Culture based and molecular detection of the microbial quality of borehole water used in some hostels in Ihiagwa, Imo State. Nigerian Journal of Microbiology, 29: 3116-3126. www.nsmjournals.org
39. Nsofor, C. A., Ogbulie, T. E. and Ugbogu, O. C. (2015). Whole Genome Sequencing: Bacterial Typing Revolutionized. Pyrex Journal of Microbiology and Biotechnology Research 1 (3):038-048. http://www.pyrexjournals.org/pjmbr
40. Ogbulie, Toochukwu E., Duru, Christopher and Nwanebu, Ferdinand C. (2015) Interaction Effects of Plants and Indigenous Micro-organisms on Degradation of N-Alkanes in Crude Oil Contaminated Agricultural Soil. Journal of Ecosystem and Ecography 5: 2-16 http://dx.doi.org/10.4172/2157-7625.1000166
41. Nsofor C.A, Anyanwu N.C and Ogbulie T.E(2016). High Antibiotic Resistance Pattern Observed in Bacterial Isolates from a Tertiary Hospital in Southeast Nigeria. International Journal of Research in Pharmacy and Biosciences. 2(7):1-6 www.ijrpb.org
42. Ogbulie T. E and Nwaokorie F.O (2016) Molecular Diversity of Microbes with Probable Degradative Genes in Agricultural Soil Contaminated with Bonny Light Crude Oil. Journal of Ecosystem and Ecography. 6: 1-9 http://dx.doi.org/10.4172/2157-7625.S5-002
43. Chikezie, U. N, Nsofor C.A, Adjeroh L.A, Ogbulie T. E, Udensi, J.U and Oyirioha K.C (2016). An Evaluation of the Phytochemical and Nutritional Compositions of Fresh Leaves of Cnidoscolus aconitifolius. International Journal of Research Studies in Biosciences 4(6) 21-28 http://dx.doi/10.20431/2349-0365.0406004
44. Anuforo Henry Uzoma, Ogbulie Tochukwu Ekwutosi, and Akujobi Campbell Onyeka (2016). Comparative Study of Effects of Electrode Materials and Catholyte on Simultaneous Generation of Bioelectricity and Wastewater Treatment. International Journal of Bioorganic Chemistry; 1(1): 8-20 http://www.sciencepublishinggroup.com/j/ijbc ;doi: 10.11648/j.ijbc.20160101.12
45. Akujobi Campbell Onyeka , Anuforo Henry Uzoma , Ogbulie Tochukwu Ekwutosi and Ezeji Ethelbert Uchechukwu (2017). Study on Generation of Bioelectricity Using Potassium Ferricyanide Electron Acceptor in Microbial Fuel Cell. Chemical and Biomolecular Engineering ; 2(1): 5-13 http://www.sciencepublishinggroup.com/j/cbe doi: 10.11648/j.cbe.20170201.12
46. Justina Ugochi Udensi, Toochukwu Ekwutosi Ogbulie, Emeka-Nwabunnia Ijeoma, Nkeiruka Uche Chikezie, Ugochukwu Mmasi Godson, Awurum Ivy Nwaku, Ifeyinwa Celestina Mgbemena (2017), Study on the Effects of Hydraulic Dredging and Disposal Operations on Water Quality of Nworie River, South-Eastern Nigeria. International Journal of Environmental Monitoring and Analysis. 5(2), 25-31. doi: 10.11648/j.ijema.20170502.12
47. Ogbulie, T. E., Anuforo, H. U. Akujobi, C., O.,Nwachukwu, A. A. and Okika, W. O (2017) Potential Exoelectrogenic Bacteria Species Isolated from Piggery Wastewater used in Generation of Bioelectricity and Wastewater Treatment. Analele Universitatii din Oradea Fascicula Biologie Tom. XXIV, Issue: 1: 30-39 http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2017-1.html
48. Anuforo, H. U., Ogbulie, T. E., Akujobi, C. O., and Ezeji, E. U(2017). Study on the Use of Microbial Fuel Cell as Waste Management Option to Generate Electricity from Piggery Wastewater . Analele Universitatii din Oradea – Fascicula Biologie Tom. XXIV, Issue: 1: 40-47. http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2017-1.html
49. Okore, C. C., L. U. Nwaehiri., O.N. Mbanefo., T. E. Ogbulie., A. U. Ugenyi., I. B. Ogbuka., A. E. Ejele and I. A. Okwujiako (2017). Study on microbial diversity of biosurfactant producing bacteria from contaminated environmental samples. International Journal of Advanced Research. 5(6 ), 1387-1396. Article DOI:10.21474/IJAR01/4548 DOI URL: http://dx.doi.org/10.21474/IJAR01/4548.
50. Okore, C. C., L. U. Nwaehiri., O.N. Mbanefo., T. E. Ogbulie., A. U. Ugenyi., I. B. Ogbuka., A. E. Ejele and I. A. Okwujiako (2017). Study on effect of monovalent and divalent salts on the production of Biosurfactant and emulsification index. International Journal of Advanced Research 5(6): 1875-1881. Article DOI:10.21474/IJAR01/4616; DOI URL: http://dx.doi.org/10.21474/IJAR01/4616
51. Okore, C. C., L. U. Nwaehiri., O.N. Mbanefo., T. E. Ogbulie., A. U. Ugenyi., I. B. Ogbuka., A. E. Ejele and I. A. Okwujiako (2017). Study on the production and Antibacterial activities of biosurfactant produced from some bacterial species. International Journal of Advanced Research 5(7 ): 581-586 . Article DOI:10.21474/IJAR01/4755; DOI URL: http://dx.doi.org/10.21474/IJAR01/4755.
52. Okore, C. C., L. U. Nwaehiri., O.N. Mbanefo., T. E. Ogbulie., A. U. Ugenyi., I. B. Ogbuka., A. E. Ejele and I. A. Okwujiako (2017). The Use Of Random Amplified Polymorphic DNA (RAPD) to Study the genetic variation of Biosurfactant Producing Bacteria. Academic Journal of Science, 07(03):267–286 ISSN: 2165-6282 . http://www.universitypublications.net/ajs/0703/pdf/K7D177
53. Ugoenyi, A U., Ozoh, P.T., Ukwandu, N.C., Mgbemena, I.C., Okore, C.C.,Onyeocha, I.O., Okereke, J.N., Ogbulie, T.E and Adjaero, L.A (2018). Acute and Sub-chronic Toxicity of Euphobia serrata Ethanol extracts in adult male Albino Rats. International Journal of Advanced Research in Biological Sciences 5(9): 164-173 ISSN: 2348-8069 DOI http://dx.doi.org/10.22192/ijarbs.2018.05.09.017
54. Ogbulie, T.E., Okore, C. C., Ejele, A. E. and Okwujiako, I. A. (2020)Comparative study on identification of biosurfactant producing bacteria using three screening tests Analele Universitatii din Oradea – Fascicula Biologie, 27(2): 179-186 http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2020-2.html;
55. Anuforo, H.U., Ogbulie, T.E., Elumezie, A.O and Nwachukwu, A. A. (2020) Impact of heavy metals on safety of cattle meat sold in Owerri metropolis, imo state, Nigeria Environmental Engineering and Management Journal, 19(11) : 2013-2019 http://www.eemj.icpm.tuiasi.ro/; http://www.eemj.eu
56. Nwachukwu, A.A. Ogbulie, T. E., Udodi, N.C., Evans-Kemka, C.I. and Onyekachi, V.C. (2020) Assessment of Proximate, Phytochemical and Selected Mineral Content of Acanthus montanus Leaf. Asian Journal of Biotechnology and Bioresource Technology 6(2): 45-54, doi: 10.9734/AJB2T/2020/v6i230079
57. Onwuakor, C.E., Ogbulie, J.N., Braide, W., Ogbulie, T.E., Nwokafor C.V. and Uchendu C.E. (2020)Optimization of culture conditions for enhanced bacteriocin production by Lactococcus lactis MT186647 using response surface methodology. American Journal of Microbiological Research, 8(4): 110-116 DOI:10.12691/ajmr-8-4-1
58. Unachukwu, M.N., Nwakanma, C., Ogbulie, T. E. and Enebechi, C. L (2020). Antimicrobial activity of formulated local herbal soap made from pawpaw (carica papaya) leaf extract. International Journal of Agriculture and Rural Development 23(2): 5211-5215.
59. Ogbulie, T.E., Okore, C. C., and Ejele, A. E., (2021).Comparative assay on heavy metals removal from soil using chemical surfactant, crude biosurfactant and the producing-bacteria. Analele Universitatii din Oradea – Fascicula Biologie, 28(1): 35-41 http://www.bioresearch.ro/revistaen.html; https://www.bioresearch.ro/2021-1.html https://www.bioresearch.ro/2021-1/035-041-AUOFB.28.1.2021-OGBULIE.T.E.-
60. Okore, Chioma C. , Ogbulie, Toochukwu E. , Ejiogu, Chris, Odangowe, Ogidi I., Duruojinkeya, P., Ogbuka, Ifeanyi and Ajoku, Joseph U. (2021).Removal of Pb (II) from Synthetic solution usingActivated Carbons produced from Agricultural wastes. Global Scientific Journals, 9(4), 2697-2707.www.globalscientificjournal.com
61. Onwuakor, C. E., Ogbulie J.N., Braide, W., Ogbulie T.E., Nwokafor, C.V., and Uchendu C.E. (2021). Optimization of Bacteriocin Production by Lactobacillus fermentumStrain COE20 from Fermenting Pentaclethramacrophylla Benth Using Response Surface Methodology. American doi:10.12691/ajfst-9-2-1
62. Ugwu, T. N., Nwachukwu, A. A.,Ogbulie T. E. and Anyalogbu, E. A. (2021). Compositional Assessment of Selected Plant-basedSubstrates for Biogas Production. Journal of Advances in Biology & Biotechnology, 24(3): 1-6. DOI:10.9734/jabb/2021/v24i330202 ; https://www.journaljabb.com/index.php/JABB/article/view/30202
63. Chioma C. Okore, Toochukwu E. Ogbulie, Ogidi I. Odangowei , Chris Ejiogu, , Prisca Duruojinkeya, IfeanyiB. Ogbuka, Joseph U. Ajoku (2021).Heavy Metals Incidence and Plasmid Profiles ofBacteria Isolated from borehole water around Federal Polytechnic Nekede Communities Global Scientific Journals, 9(6), 1247-1261.www.globalscientificjournal.com
64. Ogbulie, T.E., Opara, C.N., Nwachukwu, A.A., Anuforo, H.U. and Ojimadu, P.O (2021). Assessment of Hygiene Status of Implements for Sale of Raw Beef in SelectedRetail Shops in Umudike, Abia State, Nigeria. EBSU Science Journal, 2: 27-34.
65. Chioma C. Okore, Toochukwu E. Ogbulie, Ogidi I. Odangowei, Chris Ejiogu, Prisca Duruojinkeya, Ifeanyi B. Ogbuka, Joseph U. Ajoku (2021). Plasmid Profiles of Bacterial Isolatesfrom Kerosene, Diesel and Crude Oil Polluted Soils. Global Scientific Journals, 9(7), 1994- 2005. www.globalscientificjournal.com
66. Augusta Anuli Nwachukwu, Toochukwu Ekwutosi Ogbulie, Harriet Chinyelu Nwigwe, Ernest Anayochukwu Anyalogbu and Henry Uzoma Anuforo (2022). Effect of Fungal, Bacterial and Alkaline Augmentations on the Biogas Composition of Selected Plant-based Substrates. Asian Journal of Biotechnology and Bio resource Technology 8(3): 16-22. DOI: 10.9734/AJB2T/2022/v8i330126 https://www.sdiarticle5.com/review-history/87398
67. Tochukwu Nicholas Ugwu, Augusta Anuli Nwachukwu, Toochukwu Ekwutosi Ogbulie and Ernest Anayochukwu Anyalogbu(2022), Effective Optimization of Bacterial and Alkaline Augmented Plants Substrate on Biogas Yield Using Operational Conditions. Green Energy and Environmental Technology, 1–18. DOI: https://doi.org/10.5772/geet.12
68. R. C. Uduma, K. L. Oguzie, C. F. Chijioke, T. E. Ogbulie, E. E. Oguzie (2023). Bio-electrochemical technologies for simultaneous treatment of dye wastewater and electricity generation: A review. International Journal of Environmental Science and Technology. https://doi.org/10.1007/s13762-022-04753-0
69. Uwakwe, F. E, Ezejiofor, T. I. N, Ogbulie, T. E, Anyalogbu, E. A. A, Okafor, S. A. (2023). Investigating the Biodegradation of Low-Density Polyethylene by Proteus and Serratia spp. Engineering and Technology Journal 08 (03), 2052-2055 DOI: 10.47191/etj/v8i3.08, I.F. – 7.136 http://everant.org/index.php/etj/article/view/860/611
70. Mercy Ngozi Madubuike , Toochukwu Ekwutosi Ogbulie, Chidinma Alvana Unegbu , Janet Damilola Johnson and Chimela Emmanuel Ibe (2023). Evaluation of Total Hydrocarbon Contents in the Leaves of Selected Crops Grown on Crude Oil Polluted Agricultural Soil, Biotechnology Journal International 27(4): 26-34 DOI: 10.9734/BJI/2023/v27i4688; https://www.sdiarticle5.com/review-history/101282
71.) John O. Anyanwu, Kanayo L. Oguzie, Toochukwu E. Ogbulie, Christogonus O. Akalezi, and Emeka E. Oguzie (2023) Electrochemical and microbial treatment of bromophenol blue dye in aqueous solution. J. Electrochem. Sci. Eng.00(0) (2023) 000-000; http://dx.doi.org/10.5599/jese.1882 72.) Anene, C.E., Oguzie, K.L., Ogbulie, T.E., Akalezi, C O., and Oguzie., E. E.(2023) Electrochemical and microbial decolourization of Congo Red dye-contaminated wastewater: experimental and computational studies. Chem. Pap. . https://doi.org/10.1007/s11696-023-03045-2.
73.) Ugochi Nneka Kemka, Toochukwu Ekwutosi Ogbulie, Kanayo Oguzie, Christogonus Oudney Akalezi, and Emeka Emmanuel Oguzie (2023). Pretreatment Procedures on Lignocellulosic Biomass Material for Biogas Production: A Review. Analele Universităţii din Oradea, Fascicula Biologie. https://www.bioresearch.ro/revistaen.html
74.) Uwakwe Felicia E.1, Ezejiofor Tobias I.N., Ogbulie Toochukwu, E. & Anyalogbu Ernest A.A (2023) A Comparative Study of Biodegradation of Virgin andWeathered Low-density Polyethylene by Proteus and Lactobacillus spp. International Journal of Advanced Research in Biological Sciences 10(7): 93-102. http://dx.doi.org/10.22192/ijarbs.2023.10.07.011
75.) Onyeka CA, Ogbulie TE, Anyado- Nwadike OS, Ezeji EU, Okwujiako IA. (2023) Domestication of the wild edible mushroom, Lentinus squarrosulus (mont.). Journal of Plant Biotechnology and Microbiology, 6(4):156 https://www.alliedacademies.org/articles/domestication-of-the-wild-edible- mushroom-lentinus-squarrosulus-mont-26356.html.
76.) Nnenna Nnannaya OTI, Toochukwu Ekwutosi OGBULIEand Augusta Anuli NWACHUKWU, And Mercy Ngozi MADUBUIKE (2023) Cytotoxic Investigation of Crude Oil Impact on Selected Crops Via Pre-Planting and Post-Planting Treatments. Analele Universităţii din Oradea, Fascicula Biologie 30 (2), 159-165.
77.) Anuforo, H. U., Ogbulie, T. E, Udebuani, A. C. and Ezeji, E. U. (2023). Synthesis of Zinc Oxide Nanoparticles using Extract of Cynodon dactylon andAssessment of their Biological Activity against Staphylococcus aureus andEscherichia coli. Journal of Microbiology Research (UJMR), 8 (2), 74 – 85.
78.) B. I. Ugwu · C. Akalezi · I. S. Ike · T. E. Ogbulie and E. E. Oguzie (2024) Biomass Oxidation in Direct Biomass Redox Flow Fuel Cell: The Roles of Catalysts, Redox Couples, Mediators and Oxidants. Korean Journal of Chemical Engineering 41(1) xx https://doi.org/10.1007/s11814-024-00131
Conference Proceedings 79. Ogbulie T. E. and Ucheime, A. C. (2015). Assessment of Population dynamics of biodegradable Consortia in Bonny light crude oil polluted agricultural soil using molecular and culture-based Approach. Proceedings of the 6th World Congress on Biotechnology, OMICS International conferences, October 5-7, 2015 Rohini, Delhi, India pp 176.
80. Ogbulie, T. E, Duru, C. M and Nwanebu F. C. (2015). Study on the effect of interaction of plants and indigenous micro-organisms in degradation of n-alkanes in crude oil contaminated agricultural soil. Proceedings of the 4th International Conference on Biodiversity, OMICS International conferences, June 15-17, 2015 Las Vegas, Nevada USA pp 34.
81. Ogbulie, T. E. and Nwaokorie, F. O. (2015). Molecular diversity of microbes with probable degradative gene in agricultural soil contaminated with Bonny light crude oil. Proceedings of the 4th International Conference on Biodiversity, OMICS International conferences, June 15-17, 2015, Las Vegas, Nevada USA pp 63.
Chapters in Books in Relevant Area of research 82. Uwaezuoke, J.C. and Ogbulie. T.E (2000). Laboratory Equipments and General Techniques In: Biological and Agricultural Technique. Webs Communications, Nigeria ISBN 978-33947-8-9. Pp 1-9.
83. Uwaezuoke, J.C. and Ogbulie, T.E. (2000). General Microscopy. In: Laboratory Manual for University Biology. Egoba Associates, Owerri, Nigeria ISBN 978-34741-6-2.Pp 5-20.
84. Ogbulie, T. E (2012) Introduction to Genetics In: Foundation Biology for University Students Vol I. ApplauseB Multisectors Ltd Owerri, Nigeria. ISBN 978-219-314-3 pp199-238.
85. Ogbulie T. E. and J.N. Ogbulie (2013) Agricultural Microbiology In: Foundation Biology for University Students Vol II. ApplauseB Multisectors Ltd Owerri, Nigeria. ISBN 978- 219-354-2 pp 227-317.
86. Ogbulie, T. E. (2013). Spectrophotometric Techniques In: Research Technique in Biological and Agricultural Science. Unique Books, Webs Media Communication LTD ISBN 978-978-50638-9-9 pp 272 – 303.
87. Ogbulie, T. E. and Ogoamaka, A. I (2015). Preparation and Observation of cultures and microscopic slides In: Essential Analytical Methods in Biotechnology. . Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 pp 63 – 112.
88. Ogbulie, T. E. (2015). DNA: The basis of inheritance in organisms. In: Essential Analytical Methods in Biotechnology. . Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 pp 113 – 147.
89. Ogbulie, T. E. and Fowora, M. A. (2015). The Chromosomes, Plasmid DNA, Phages and their general Extraction Methods. In: Essential Analytical Methods in Biotechnology. Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 pp 158 – 212.
90. Ogbulie, T. E. and Nwaokorie, F. O. (2015). Genetic Modification in Biotechnology. In: Essential Analytical Methods in Biotechnology. Unique Books, Webs Media Communication LTD ISBN 978-978- 52586-4-6 pp 213- 264.
91. Ogbulie, T. E. (2015). Principles, Methods and Application of Gel Electrophoresis. In: Essential Analytical Methods in Biotechnology.Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 pp 265- 314.
92. Ogbulie, T. E., Nwakanma, C and Anuforo, H. U. (2015). Biological tool used in Biotechnology. In: Essential Analytical Methods in Biotechnology.Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 pp 357- 450.
93. Ogbulie, Toochukwu Ekwutosi, Opara, Christiana N , Nwachukwu, Augusta Anuli and Ogbodo, Priscilla Amaka(2022). Strategies for yeast strainimprovement through metabolic Engineering In:Multidisciplinary Applications and Advances in Biotechnology. Vol 1 Ch 7 pp 119-142. Taylor and Francis group, USA. DOI https://doi.org/10.1201/9781003178378 https://www.taylorfrancis.com/chapters/edit/10.1201/9781003178378-7/strategies-yeast- strain-improvement-metabolic-engineering-toochukwu-ekwutosi-ogbulie-augusta-anuli- nwachukwu-priscilla-amaka-ogbodo-christiana-opara?context=ubx&refId=d55d1177-2ceb- 47ac-94c6-eead32c29812 https://www.routledge.com/Multidisciplinary-Applications-and-Advances-in-Biotechnology- Contributions/Ubi-Uzochukwu-Nwoba-Ogbonna-Adetunji-Esiobu-Obembe-Ibrahim-Ekundayo- Okoli-Gidado-Inuwa-Ezeonu-Christpeace-Popoola/p/book/9780367760465
94. Toochukwu E. Ogbulie, Nwadiuto Esiobu and Muinah Foworah (2022) Development and composition of the human microbiome from birth In: Multidisciplinary Applications and Advances in Biotechnology. Vol 5 Ch 4. pp 67 – 78, Taylor and Francis group, USA. https://doi.org/10.1201/9781003180241 https://www.taylorfrancis.com/chapters/edit/10.1201/9781003180241-4/development- composition-human-microbiome-birth-toochukwu-ogbulie-nwadiuto-diuto-esiobu-muinah- fowora?context=ubx&refId=3f3333af-b29f-436a-8297-5a0ac7119847 https://www.routledge.com/Multidisciplinary-Applications-and-Advances-in-Biotechnology- Contributions/Ubi-Uzochukwu-Nwoba-Ogbonna-Adetunji-Esiobu-Obembe-Ibrahim-Ekundayo- Okoli-Gidado-Inuwa-Ezeonu-Christpeace-Popoola/p/book/9780367760465
95. Toochukwu E. Ogbulie, Nwadiuto Esiobu and Ifeoma Enweani (2022) A Brief Review of Earth Microbiomes and Applications. In: Multidisciplinary Applications and Advances in Biotechnology. Vol 5 Ch 8. pp 137- 160, Taylor and Francis group, USA. https://doi.org/10.1201/9781003180241 https://www.taylorfrancis.com/chapters/edit/10.1201/9781003180241-8/brief-review-earth- microbiomes-applications-toochukwu-ogbulie-nwadiuto-diuto-esiobu-ifeoma-enweani- nwokelo?context=ubx&refId=503e4881-a599-4053-a348-703e00d7d5fd https://www.routledge.com/Multidisciplinary-Applications-and-Advances-in-Biotechnology- Contributions/Ubi-Uzochukwu-Nwoba-Ogbonna-Adetunji-Esiobu-Obembe-Ibrahim-Ekundayo- Okoli-Gidado-Inuwa-Ezeonu-Christpeace-Popoola/p/book/9780367760465
Editor/ Co-Editor of Textbook in Relevant Area of research
96. Ogbulie, T. E., Ezeji, E. U. and Nsofor, C. A. (2014). A concise guide to in-vitro recombinant DNA technology: Laboratory manual Unique books, Webs Media Communications LTD Owerri Nigeria ISBN978-978-52588-5-6, 56 p.
97. Ogbulie, T. E. and Nwakanma, C (2015) Essential Analytical Methods in Biotechnology. Unique Books, Webs Media Communication LTD ISBN 978-978-52586-4-6 464p.
98.Ogbulie, T. E. (2015). Concise guide to Experimentations in Molecular biology: Laboratory Manual, Stanet Technologies Owerri, ISBN 978-25797-5-3, 67p.
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